Exhibit 16

April 4, 2025

Securities and Exchange Commission

100 F Street, N.E.

Washington, DC 20549

Commissioners:

We have read the statements made by Escalon Medical Corp. under Item 4.01 of its Form 8-K dated April 2, 2025. We agree with the statements concerning our Firm in such Form 8-K; we are not in a position to agree or disagree with other statements of Escalon Medical Corp. contained therein.