EX-23.1

EX-23.1 4 iobt-ex23_1.htm EX-23.1 EX-23.1

Exhibit 23.1

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in the following Registration Statements:

(1)

Registration Statement (Form S-3 No. 333-269569) of IO Biotech, Inc.,

(2)

Registration Statement (Form S-8 No. 333-262587) pertaining to the 2021 Equity and Incentive Plan and 2021 Employee Stock Purchase Plan of IO Biotech, Inc.,

(3)

Registration Statement (Form S-8 No. 333-269597) pertaining to the IO Biotech, Inc. 2021 Equity and Incentive Plan,

(4)

Registration statement (Form S-3 No. 333-274267) of IO Biotech, Inc., and

(5)

Registration Statement (Form S-8 No. 333-277826) pertaining to the IO Biotech, Inc. 2021 Equity and Incentive Plan, the IO Biotech, Inc. 2023 Inducement Award Plan, and the Option Inducement Award;

of our report dated March 31, 2025, with respect to the consolidated financial statements of IO Biotech, Inc. included in this Annual Report (Form 10-K) of IO Biotech, Inc. for the year ended December 31, 2024.

/s/ EY Godkendt Revisionspartnerselskab

Copenhagen, Denmark

March 31, 2025