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Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statement on Form S-8 (No. 333-239571) of Akouos, Inc. of our report dated March 29, 2021 relating to the financial statements, which appears in this Form 10-K.

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/s/PricewaterhouseCoopers LLP

Boston, Massachusetts

March 29, 2021